![]() ![]() NTA appears to be more convenient to use, but still has certain drawbacks: for example, two populations can only be resolved if their particle diameters differ at least by a factor 1.5. It was argued that EM underestimates the size of vesicles in addition, the real size distribution of the entire vesicle population is not assessable by this method. Several other techniques were tested in the past for size determination of EVs isolated from blood, for example scanning or transmission electron microscopy (EM) nanoparticle tracking analysis (NTA), and flow cytometry (FC). However, further development of the methodology is required. These results show that SLS and DLS are promising methods for the analysis of morphological features of ENPs and have the potential to discriminate between OC and BP patients. The R h and R g of ENPs in BP patients were larger than in OC patients, with ρ ≈ 1.1–2, implying a more elongated/distorted shape. The shape parameter ρ of both particle populations was around 1, which is typical for spherical particles with mass concentrated on the rim, as in vesicles. In thawed samples, larger particles ( R h mostly above 100 nm) were detected as well. R h and R g of the predominant ENP population of OC patients were in the range 20–30 nm (diameter 40–60 nm). The hydrodynamic radius ( R h) and the radius of gyration ( R g) of ENPs were calculated from the angular dependency of LS intensity for two ENP subpopulations. Samples of plasma and ascites (OC patients) or plasma, peritoneal fluid, and peritoneal washing (BP patients) were analyzed. ![]() In this contribution, the use of non-invasive static (SLS) and dynamic light scattering (DLS) for the characterization of extracellular nanoparticles (ENPs) in body fluids of advanced serous ovarian cancer (OC) and benign gynecological pathology (BP) patients is demonstrated and critically evaluated. In parallel to medical treatment of ovarian cancer, methods for the early detection of cancer tumors are being sought. ![]()
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